Media:
1. Media suitable for the growth of Pichia pastoris was prepared. For this culture Tryptone Soya Broth (TSB, 30g/L) media was used.
2. For Shaker Flask a total of 300ml TSB media was prepared, divided into 75 * 4 ml, taken in 500ml flask to provide adequate headspace for the growth of microbes.
3. For 5l Fermenter 3l of TSB media was prepared.
4. The pH of the media was adjusted to 5.0 as this is the optimal pH for growth of Pichia pastoris.
Antifoam:
1. 25% Silicon antifoam was prepared to control the foam formation in the 5l fermenter.
Acid & Base preparation:
1. 2N HCl & 2N NaOH were prepared to adjust the pH.