To get the same results as in lab scale these following parameters need to be kept constant:
- Column Length
- Linear Flow Rate
- Ratio Between Gradient and Column Length
Constant Column Length
Desired Capacity should be proportional to the cross sectional area of the column, because same proportion of the column should be occupied by the samples on both the scales
Gradient
Gradient volume is decided when the diameter of the column is set. Gradient elution in process scale will take the same time as in laboratory scale.
Sometimes a lower flow rate will compensate the decrease in resolution due to ineffective solvent distribution.
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