Column chromatography is technique used to separate compounds of interest from the others using a column finely packed with media/matrix as stationary phase.

Matrix used should have certain properties these includes:
1. Mechanical Stability
2. Chemical Stability
3. Should be chemically inert

Commonly Used Matrices

Cellulose Based :
Eg: Cellufine

Dextran Based :
Eg: Sephadex, Sephacryl

Agarose Based :
Eg: Sepharose, Biogel A

Polyacrylamide Based :
Eg: Biogel – P

Polystyrene Based :
Eg: Dowex – 50

Silica Based :

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