This course will provide you with a comprehensive understanding of qPCR and its applications as a diagnostic tool. We'll explore the theoretical foundations of the technique, its practical implementation, and its various diagnostic applications.
The courses is divided into 3 modules, Our first module, "Understanding qPCR Basics," will lay the groundwork for your learning. We'll delve into the fundamental principles of qPCR.
Building upon this foundation, Module 2, "qPCR Methodology and Optimization," will guide you through the practical aspects of the technique. We'll cover: Primer designing consideration, qPCR assay optimization, etc.
Now, we'll shift our focus to the advances in qPCR in Module 3, "advanced topics in qPCR." We'll explore new and emerging technologies used to diagnose various diseases.
This module will also provide you with real-world applications like use of qPCR in the areas of detection of Infectious diseases, cancer diagnostics and in the areas of monitoring efficiency of drug treatment by looking at the gene expression.
Course Content
The qPCR Diagnostic Mastery Course : Module 1
1.1 What is qPCR and how does it work?
1.1.1 Basic Principles of PCR:
1.1.2 Key Differences Between qPCR and Conventional PCR:
1.1.3 Fluorescent Detection and Quantification:
1.1.4 Different Types of qPCR Assays:
1.2 Applications of qPCR in Molecular Diagnostics
1.2.1 Use of qPCR in Various Areas:
1.2.2 Advantages and Disadvantages: Advantages:
1.3 Interpretation of qPCR Data
1.3.1 Importance of Ct Values:
1.3.2 Standard Curves and Data Analysis:
1.3.3 Data Normalization and Relative Quantification:
