TAE Buffer- Tris Acetic EDTA Buffer
TAE Buffer consists of Tris Base, Acetic acid and EDTA, a buffer which is most commonly used as running buffer for agarose gel electrophoresis in the separation of nucleic acids (DNA and RNA).
Preparation of 50X TAE Buffer Stock Solution
Chemical
|
Weight / Volume
|
Molarity
|
Tris Base
|
242 gms
|
2 Molar
|
Acetic Acid
|
57.1mL
|
|
0.5M EDTA pH 8.0
|
100 mL
|
50 milli Molar
|
MilliQ
|
up to 1000 mL
|
|
Final Concentration in 1X TAE Buffer
Tris – 40mM
EDTA – 1mM
Preparation 1X TAE From 50X TAE Stock
This can be calculated using the formula:
C1*V1 = C2*V2
C1 – Initial Concentration
V1 – Initial Volume
C2 – Final Concentration
V2– Final Volume
For Example, you want to make 1X TAE Buffer from 50X TAE Buffer Stock then how much 50X TAE is required,
50 * V1 = 1*300
V1 = 300 / 50 = 6mL
So to make 300mL of 1X TAE Buffer you should take 6mL of 50X TAE Stock solution and make it up to 300mL using MilliQ water (6mL of 50X TAE + 294mL of Water).
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