TAE Buffer- Tris Acetic EDTA Buffer TAE Buffer consists of Tris Base, Acetic acid and EDTA, a buffer which is most commonly used as running buffer for agarose gel electrophoresis in the separation of nucleic acids (DNA and RNA). Preparation of 50X TAE Buffer Stock Solution Chemical Weight / Volume Molarity Tris Base 242 gms 2 Molar Acetic Acid 57.1mL 0.5M EDTA pH 8.0 100 mL 50 milli Molar MilliQ up to 1000 mL Final Concentration in 1X TAE Buffer Tris – 40mM EDTA - 1mM Preparation 1X TAE From 50X TAE Stock This can be calculated using the formula: C1*V1 = C2*V2 C1 - Initial Concentration V1 - Initial Volume C2 - Final Concentration V2- Final Volume For Example, you want to make 1X TAE Buffer from 50X TAE Buffer Stock then how much 50X TAE is required, 50 * V1 = 1*300 V1 = 300 / 50 = 6mL So to make 300mL of 1X TAE Buffer you should take 6mL of 50X TAE Stock solution and make it up to 300mL using MilliQ water (6mL of 50X TAE + 294mL of Water). Got something to say about this post? Leave a comment...your comments are valuable for improving the posts.