A good note on Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for
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Primer and Probe Design for Real Time PCR – Quantitative Assays:
Primer and probe designing softwares are available online which can aid in easy designing of
Real time qPCR vs Digital PCR
Detection of low levels of pathogen Low level detection of pathogens can be done using
Real Time PCR (qpCR) Quantitation – Methods and Types of analysis – Absolute vs Relative Quantitation
A typical real time PCR graph – qPCR graph showing Ct (cycle threshold) or Cq
Conversion and Calculations of Nucleic Acids and Proteins
Molecular Weights (MW) Average MW RNA nucleotide = 340 DaltonsMW ss RNA = (# nucleotides)